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Instructor Resources - Macmillan LearningThey are kept in place with special hypoallergenic adhesive tape. The patches stay in place undisturbed for 48 hours. At the second appointment, usually two days later, the patches will be removed. Sometimes further patches are applied. The back is marked with an indelible black felt tip pen or other suitable marker to identify the test sites. These marks must still be visible at the third appointment, usually two days later (4 days after application). The back should be checked and if necessary remarked on several occasions between the 2nd.